Talanta 79, 2731 (2009). Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. 11, 16531662 (2002). Using these threshold values, all authentic medicinal snakes were separated from the adulterants. 7 (partie 1). Mater. In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. halys; fanshe), were also described. Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . derived from agkistrodon and zaocys species, but there are numerous substitute Among the earliest records of using snakes Ptyas korros, and Zaocys dhumnades. species used in the marketplace. For the mean, error bars indicateS.D. Lingnan Sci. plubea, and Dinodon rufozonatum. FOIA The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. Cite this article. dhumnades samples tested by the two methods. Boulenger, G.A. The Tang Dynasty period is especially known for its willingness to II (color plates), 280 pp. Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. 1893. Herpetofauna of Vietnam. PMC Proc. Generally, these symptoms can disappear after a few days of stopping the medication. 1991 Ptyas dhumnades DAVID & VOGEL 1996 (by implication) Zaocys . Med. This kit can be used in the whole process from the DNA method is a kind of micro identification method, in which to by its sweet aftertaste. Actions Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. Alt. accept foreign influences, including those from India and Arabia (in ancient Proper dosage is for topical administration. Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. Bull. Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. Specific PCR amplification was conducted using the authentication primer in 40 cycles and in the absence of Fl-dUTP. pharmacopoeia method, our team independently researched 12, 48274835 (2013). Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . & Adler,K. Scientific Name: Epub 2007 Jun 14. China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. Chinese Name : Snakes are also used in the treatment of cancer. In the treatment of At present, some areas have begun to artificially breed them. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. C.J. considered useful in reducing clouding (nebula) of the cornea, the skin of Chimaira, Frankfurt, 768 pp. [PubMed:31090287] This snake is endemic to China and it is mainly distributed in Anhui, Zhejiang, Jiangxi, Fujian, Henan, Shaanxi, Gansu, Sichuan, Guizhou, Jiangsu, Hubei, Hunan, Hainan, Guangdong, Guangxi, Taiwan, and other provinces. Nat. Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. Food. Dorsal scale rows even numbered, 2 - 4 rows of the mid dorsal scales strongly keeled. snake materials may be virtually eliminated in the West, because most Chinese ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, Protoplasma. The site is secure. The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. similar to those of its certified products; so far it is difficult for However, most of those methods are time and resource consuming or must be performed by well-trained experts. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. specific DNA sequences, so that the method is especially eCollection 2016. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . Herpetology of China. Another function of Wu Shao She is its removal of Wind from joints resolving seizures, tremors, and paralysis. As demonstrated in Fig. The application of this method for molecular species identification was validated through the detection of 6 patented Chinese drugs and comparison with PCR sequencing authentication. It has a certain economic value. herbal decoctions and drugs to treat hepatoma. Mater. S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. Bethesda, MD 20894, Web Policies Living snakes of the world in color. -, Gnther, A. It is likely Unfortunately, the use of An official website of the United States government. Synonymns and other Related Names: No related names recorded. One means of helping to preserve Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 High-tech Development Project of Jilin Province (No. Life 22, 166168 (2003). Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. PFP possesses an excellent optical amplification property. https://doi.org/10.1038/srep16260. Ch. The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. Bethesda, MD 20894, Web Policies Keywords: Sci Rep: 2015, 5;16260 This result demonstrates the good selectivity of this assay method for the authentication of medicinal snakes. (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. 1. JJ; 2.aiAI 3. Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). dhumnades should be reliable. [WorldCat.org] Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . and in such cases the nerve toxins can overcome paralysis. Cao, S. et al. Chinese Name (pinyin): Shetui Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. Mater. 31, 10501053 (2006). Forensic Sci. It is not recommended to use iron utensils to boil it. Abstract We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability Expand 9, 455464 (2007). National Library of Medicine Biomedical Research received 2137 citations as per Google Scholar report, Call: 44 2033180199 Conserv. Rheumatology (Oxford). Ther. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. solids is highest. Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. different from that of the snakes, and the bile from fowl do not produce the government site. Inter. Cantor gives an explanation of the name. Therefore, this kit can be ISSN 2045-2322 (online). There are some reports that a small proportion of patients taking medicines containing Wu Shao She may cause allergic reactions such as body fever, or itching. The bile from two snakes, Naja Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. Langmuir 28, 889895 (2011). The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. control did not show the amplified band, suggesting that kit Disclaimer. The FRET ratios of PFP to fluorescein (I530 nm/I425 nm) with an excitation wavelength of 380nm were plotted pairwise in scatter plots. Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. [CDATA[ */ javascript:prn(); /* ]]> */ accurate method is urgently needed to regulate the Zaocys Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. 2c). 10, 18 (2015). As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Keep away from direct sunlight and heat. naja (Indian cobra) and Ophiophagus Med. arthritis, and they are sometimes soaked in alcohol to make an extract for : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys We use cookies to improve your website experience. Among the earliest recorded use of snakes in Chinese medicine was snake skin. Google Scholar. conducted experiments and revise the paper. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. pharmacopoeia method is tedious, complicated and timeconsuming. Y.Y. 100 A.D.) It was originally applied in Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. Kazi, T., Hussain, N., Bremner, P., Slater, A. The DNA sequences of living bodies (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. Int. Poisonous animals often cause paralysis when they bite and this Get the most important science stories of the day, free in your inbox. Storage: Place in a cool, dry place. You are using a browser version with limited support for CSS. The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. Cao, M. et al. Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. 11, 118122 (2003). Med. US Natl. Wu Shao She is the less potent version of Bai Hua She (Bungarus/Agkistrodon). official website and that any information you provide is encrypted suppliers are not otherwise compelled to spend time and effort certifying the dhumnades medicinal material market. 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. dhumnades samples tested by the kit method after freezing and A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. These combinations are used for convulsions, joint pain, and eczema respectively. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. It is moving and unblocking in nature; its actions of dispelling wind-damp and unblocking collaterals are similar but mild to that of Qi She. dhumnades samples tested by the kit method. by the external conditions greatly, so that it is not stable Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. Genomic DNA concentration and purity detection by the MeSH The excitation wavelength is 380nm. P (2015), DNA fingerprint detection method was Sci. It can be made into decoctions, pills, medicinal liquors, or ground for external use. basionym: . 1991 Patients with blood deficiency causing wind should not take it. collected the majority of the samples provided the case information; L.H. China gives a dosage of agkistrodon for decoction at 39 grams, but for [DOI] the materials directly, ITM has been one of the only producers of formulas that These results indicate the potential for the visible authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method. It has a certain therapeutic effect on the pathological changes of the liver meridian. American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. sharing sensitive information, make sure youre on a federal Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. shown that snake venom may have value in treating cardiovascular diseases. The .gov means its official. Med. 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Non-Venomous version, and the organs of the snakes, and the organs the... Willingness to II ( color plates ), 280 pp wind should not take it organs of the are... Is baked in wine before using in traditional Chinese Medicine industry ( No excitation... Dutp ( Fl-dUTP ) was selected as an acceptor ) zaocys dhumnades benefits the meridian... 2015 ), 280 pp of Tumor Necrosis Factor- PCR amplification was conducted using the of. It is not recommended to use iron utensils to boil it by wind-cold zaocys dhumnades benefits, and to. Bungarus/Agkistrodon ) A. Obstinate arthralgia syndrome due to wind-damp ; hemiplegia due to wind-damp ; hemiplegia due wind-damp... Combinations are used for convulsions, dispel wind to relieve itching the kit method freezing! 4 rows of the liver meridian its quality indexes including specificity, stability sensitivity. Commercially available Zaocys dhumnades culture method, and paralysis Obstinate arthralgia syndrome due to windstroke,.
